How to Troubleshoot Recombinant Protein Production Issues?
If you work in biotechnology or pharmaceutical research, you already know that recombinant protein production can be both rewarding and frustrating. One week you are celebrating high yields and clean SDS-PAGE bands. The next week, expression drops, inclusion bodies form, or purification fails. When issues arise, you need a structured, practical approach to restore performance quickly and protect your timelines. This guide walks you step-by-step through how to troubleshoot recombinant protein production issues effectively—so you can move from confusion to clarity with confidence. Start by Diagnosing the Root Cause Before changing conditions randomly, pause and evaluate the full workflow. Problems in recombinant protein production typically fall into one of four categories: Low or no expression Poor solubility or inclusion bodies Protein degradation Low purity or recovery during purification Your goal is to isolate where the failure begins—cloning, ...